Sub-Compartmental Organization of Golgi-Resident N-Glycan Processing Enzymes in Plants

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Sub-Compartmental Organization of Golgi-Resident N-Glycan Processing Enzymes in Plants

In all eukaryotes, the Golgi apparatus is the main site of protein glycosylation. It is widely accepted that the glycosidases and glycosyltransferases involved in N-glycan processing are found concentrated within the Golgi stack where they provide their function. This means that enzymes catalyzing early steps in the processing pathway are located mainly at the cis-side, whereas late-acting enzy...

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Time-resolved fluorescence imaging reveals differential interactions of N-glycan processing enzymes across the Golgi stack in planta.

N-Glycan processing is one of the most important cellular protein modifications in plants and as such is essential for plant development and defense mechanisms. The accuracy of Golgi-located processing steps is governed by the strict intra-Golgi localization of sequentially acting glycosidases and glycosyltransferases. Their differential distribution goes hand in hand with the compartmentalizat...

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Golgi complexes (Golgi) play important roles in the development and function of neurons [1-3]. Not only are Golgi present in the neuronal soma (somal Golgi), they also exist in the dendrites as Golgi outposts [4-7]. Previous studies have shown that Golgi outposts serve as local microtubule-organizing centers [8] and secretory stations in dendrites [6, 9]. It is unknown whether the structure and...

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GolgiP: prediction of Golgi-resident proteins in plants

UNLABELLED We present a novel Golgi-prediction server, GolgiP, for computational prediction of both membrane- and non-membrane-associated Golgi-resident proteins in plants. We have employed a support vector machine-based classification method for the prediction of such Golgi proteins, based on three types of information, dipeptide composition, transmembrane domain(s) (TMDs) and functional domai...

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ژورنال

عنوان ژورنال: Molecular Plant

سال: 2011

ISSN: 1674-2052

DOI: 10.1093/mp/ssq082